SuicidDentistV1, Main, Exploration, bibRecord, 000084

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation.

Identifieur interne : 000084 ( Main/Exploration ); précédent : 000083; suivant : 000085

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation.

Auteurs : Leopoldina D F. Almeida [Brésil, Portugal] ; Pedro S. Babo [Portugal] ; Cristiana R. Silva [Portugal] ; Márcia T. Rodrigues [Portugal] ; Josimeri Hebling [Brésil] ; Rui L. Reis [Portugal] ; Manuela E. Gomes [Portugal]

Source :

Journal of materials science. Materials in medicine [ 1573-4838 ] ; 2018.

RBID : pubmed:29904797

Descripteurs français

KwdFr :
- Acide hyaluronique (composition chimique), Analyse de profil d'expression de gènes (MeSH), Calcium (composition chimique), Cellules souches (cytologie), Chimiotaxie (MeSH), Cytométrie en flux (MeSH), Dent (cytologie), Différenciation cellulaire (MeSH), Humains (MeSH), Hydrogels (composition chimique), Ingénierie tissulaire (MeSH), Ostéogenèse (MeSH), Phosphatase alcaline (métabolisme), Photochimie (MeSH), Plaquettes (cytologie), Prolifération cellulaire (MeSH), Pulpe dentaire (cytologie), Réactifs réticulants (composition chimique), Régénération (MeSH).
MESH :
- composition chimique : Acide hyaluronique, Calcium, Hydrogels, Réactifs réticulants.
- cytologie : Cellules souches, Dent, Plaquettes, Pulpe dentaire.
- métabolisme : Phosphatase alcaline.
- Analyse de profil d'expression de gènes, Chimiotaxie, Cytométrie en flux, Différenciation cellulaire, Humains, Ingénierie tissulaire, Ostéogenèse, Photochimie, Prolifération cellulaire, Régénération.

English descriptors

KwdEn :
- Alkaline Phosphatase (metabolism), Blood Platelets (cytology), Calcium (chemistry), Cell Differentiation (MeSH), Cell Proliferation (MeSH), Chemotaxis (MeSH), Cross-Linking Reagents (chemistry), Dental Pulp (cytology), Flow Cytometry (MeSH), Gene Expression Profiling (MeSH), Humans (MeSH), Hyaluronic Acid (chemistry), Hydrogels (chemistry), Osteogenesis (MeSH), Photochemistry (MeSH), Regeneration (MeSH), Stem Cells (cytology), Tissue Engineering (MeSH), Tooth (cytology).
MESH :
- chemical , chemistry : Calcium, Cross-Linking Reagents, Hyaluronic Acid, Hydrogels.
- chemical , metabolism : Alkaline Phosphatase.
- cytology : Blood Platelets, Dental Pulp, Stem Cells, Tooth.
- Cell Differentiation, Cell Proliferation, Chemotaxis, Flow Cytometry, Gene Expression Profiling, Humans, Osteogenesis, Photochemistry, Regeneration, Tissue Engineering.

Abstract

The restoration of dentine-pulp complex remains a challenge for dentists; nonetheless, it has been poorly addressed. An ideal system should modulate the host response, as well as enable the recruitment, proliferation and differentiation of relevant progenitor cells. Herein was proposed a photocrosslinkable hydrogel system based on hyaluronic acid (HA) and platelet lysate (PL). PL is a cocktail of growth factors (GFs) and cytokines involved in wound healing orchestration, obtained by the cryogenic processing of platelet concentrates, and was expected to provide the HA hydrogels specific biochemical cues to enhance pulp cells' recruitment, proliferation and differentiation. Stable HA hydrogels incorporating PL (HAPL) were prepared after photocrosslinking of methacrylated HA (Met-HA) previously dissolved in PL, triggered by the Ultra Violet activated photoinitiator Irgacure 2959. Both the HAPL and plain HA hydrogels were shown to be able to recruit cells from a cell monolayer of human dental pulp stem cells (hDPSCs) isolated from permanent teeth. The hDPCs were also seeded directly over the hydrogels (5 × 10⁴ cells/hydrogel) and cultured in osteogenic conditions. Cell metabolism and DNA quantification were higher, in all time-points, for PL supplemented hydrogels (p < 0,05). Alkaline phosphatase (ALPL) activity and calcium quantification peaks were observed for the HAPL group at 21 days (p < 0,05). The gene expression for ALPL and COLIA1 was up-regulated at 21 days to HAPL, compared with HA group (p < 0,05). Within the same time point, the gene expression for RUNX2 did not differ between the groups. Overall, data demonstrated that the HA hydrogels incorporating PL increased the cellular metabolism and stimulate the mineralized matrix deposition by hDPSCs, providing clear evidence of the potential of the proposed system for the repair of damaged pulp/dentin tissue and endodontics regeneration.

DOI: 10.1007/s10856-018-6088-7
PubMed: 29904797

Affiliations:

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Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en">Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation.</title>
<author><name sortKey="Almeida, Leopoldina D F" sort="Almeida, Leopoldina D F" uniqKey="Almeida L" first="Leopoldina D F" last="Almeida">Leopoldina D F. Almeida</name>
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<author><name sortKey="Reis, Rui L" sort="Reis, Rui L" uniqKey="Reis R" first="Rui L" last="Reis">Rui L. Reis</name>
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<country xml:lang="fr">Portugal</country>
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<series><title level="j">Journal of materials science. Materials in medicine</title>
<idno type="eISSN">1573-4838</idno>
<imprint><date when="2018" type="published">2018</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkaline Phosphatase (metabolism)</term>
<term>Blood Platelets (cytology)</term>
<term>Calcium (chemistry)</term>
<term>Cell Differentiation (MeSH)</term>
<term>Cell Proliferation (MeSH)</term>
<term>Chemotaxis (MeSH)</term>
<term>Cross-Linking Reagents (chemistry)</term>
<term>Dental Pulp (cytology)</term>
<term>Flow Cytometry (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Hyaluronic Acid (chemistry)</term>
<term>Hydrogels (chemistry)</term>
<term>Osteogenesis (MeSH)</term>
<term>Photochemistry (MeSH)</term>
<term>Regeneration (MeSH)</term>
<term>Stem Cells (cytology)</term>
<term>Tissue Engineering (MeSH)</term>
<term>Tooth (cytology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide hyaluronique (composition chimique)</term>
<term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Calcium (composition chimique)</term>
<term>Cellules souches (cytologie)</term>
<term>Chimiotaxie (MeSH)</term>
<term>Cytométrie en flux (MeSH)</term>
<term>Dent (cytologie)</term>
<term>Différenciation cellulaire (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Hydrogels (composition chimique)</term>
<term>Ingénierie tissulaire (MeSH)</term>
<term>Ostéogenèse (MeSH)</term>
<term>Phosphatase alcaline (métabolisme)</term>
<term>Photochimie (MeSH)</term>
<term>Plaquettes (cytologie)</term>
<term>Prolifération cellulaire (MeSH)</term>
<term>Pulpe dentaire (cytologie)</term>
<term>Réactifs réticulants (composition chimique)</term>
<term>Régénération (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Calcium</term>
<term>Cross-Linking Reagents</term>
<term>Hyaluronic Acid</term>
<term>Hydrogels</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkaline Phosphatase</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acide hyaluronique</term>
<term>Calcium</term>
<term>Hydrogels</term>
<term>Réactifs réticulants</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Cellules souches</term>
<term>Dent</term>
<term>Plaquettes</term>
<term>Pulpe dentaire</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Blood Platelets</term>
<term>Dental Pulp</term>
<term>Stem Cells</term>
<term>Tooth</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phosphatase alcaline</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Differentiation</term>
<term>Cell Proliferation</term>
<term>Chemotaxis</term>
<term>Flow Cytometry</term>
<term>Gene Expression Profiling</term>
<term>Humans</term>
<term>Osteogenesis</term>
<term>Photochemistry</term>
<term>Regeneration</term>
<term>Tissue Engineering</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Chimiotaxie</term>
<term>Cytométrie en flux</term>
<term>Différenciation cellulaire</term>
<term>Humains</term>
<term>Ingénierie tissulaire</term>
<term>Ostéogenèse</term>
<term>Photochimie</term>
<term>Prolifération cellulaire</term>
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<front><div type="abstract" xml:lang="en">The restoration of dentine-pulp complex remains a challenge for dentists; nonetheless, it has been poorly addressed. An ideal system should modulate the host response, as well as enable the recruitment, proliferation and differentiation of relevant progenitor cells. Herein was proposed a photocrosslinkable hydrogel system based on hyaluronic acid (HA) and platelet lysate (PL). PL is a cocktail of growth factors (GFs) and cytokines involved in wound healing orchestration, obtained by the cryogenic processing of platelet concentrates, and was expected to provide the HA hydrogels specific biochemical cues to enhance pulp cells' recruitment, proliferation and differentiation. Stable HA hydrogels incorporating PL (HAPL) were prepared after photocrosslinking of methacrylated HA (Met-HA) previously dissolved in PL, triggered by the Ultra Violet activated photoinitiator Irgacure 2959. Both the HAPL and plain HA hydrogels were shown to be able to recruit cells from a cell monolayer of human dental pulp stem cells (hDPSCs) isolated from permanent teeth. The hDPCs were also seeded directly over the hydrogels (5 × 10<sup>4</sup>
 cells/hydrogel) and cultured in osteogenic conditions. Cell metabolism and DNA quantification were higher, in all time-points, for PL supplemented hydrogels (p < 0,05). Alkaline phosphatase (ALPL) activity and calcium quantification peaks were observed for the HAPL group at 21 days (p < 0,05). The gene expression for ALPL and COLIA1 was up-regulated at 21 days to HAPL, compared with HA group (p < 0,05). Within the same time point, the gene expression for RUNX2 did not differ between the groups. Overall, data demonstrated that the HA hydrogels incorporating PL increased the cellular metabolism and stimulate the mineralized matrix deposition by hDPSCs, providing clear evidence of the potential of the proposed system for the repair of damaged pulp/dentin tissue and endodontics regeneration.</div>
</front>
</TEI>
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<DateCompleted><Year>2018</Year>
<Month>10</Month>
<Day>22</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>14</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1573-4838</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>29</Volume>
<Issue>6</Issue>
<PubDate><Year>2018</Year>
<Month>Jun</Month>
<Day>14</Day>
</PubDate>
</JournalIssue>
<Title>Journal of materials science. Materials in medicine</Title>
<ISOAbbreviation>J Mater Sci Mater Med</ISOAbbreviation>
</Journal>
<ArticleTitle>Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation.</ArticleTitle>
<Pagination><MedlinePgn>88</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s10856-018-6088-7</ELocationID>
<Abstract><AbstractText>The restoration of dentine-pulp complex remains a challenge for dentists; nonetheless, it has been poorly addressed. An ideal system should modulate the host response, as well as enable the recruitment, proliferation and differentiation of relevant progenitor cells. Herein was proposed a photocrosslinkable hydrogel system based on hyaluronic acid (HA) and platelet lysate (PL). PL is a cocktail of growth factors (GFs) and cytokines involved in wound healing orchestration, obtained by the cryogenic processing of platelet concentrates, and was expected to provide the HA hydrogels specific biochemical cues to enhance pulp cells' recruitment, proliferation and differentiation. Stable HA hydrogels incorporating PL (HAPL) were prepared after photocrosslinking of methacrylated HA (Met-HA) previously dissolved in PL, triggered by the Ultra Violet activated photoinitiator Irgacure 2959. Both the HAPL and plain HA hydrogels were shown to be able to recruit cells from a cell monolayer of human dental pulp stem cells (hDPSCs) isolated from permanent teeth. The hDPCs were also seeded directly over the hydrogels (5 × 10<sup>4</sup>
 cells/hydrogel) and cultured in osteogenic conditions. Cell metabolism and DNA quantification were higher, in all time-points, for PL supplemented hydrogels (p < 0,05). Alkaline phosphatase (ALPL) activity and calcium quantification peaks were observed for the HAPL group at 21 days (p < 0,05). The gene expression for ALPL and COLIA1 was up-regulated at 21 days to HAPL, compared with HA group (p < 0,05). Within the same time point, the gene expression for RUNX2 did not differ between the groups. Overall, data demonstrated that the HA hydrogels incorporating PL increased the cellular metabolism and stimulate the mineralized matrix deposition by hDPSCs, providing clear evidence of the potential of the proposed system for the repair of damaged pulp/dentin tissue and endodontics regeneration.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Almeida</LastName>
<ForeName>Leopoldina D F</ForeName>
<Initials>LDF</Initials>
<AffiliationInfo><Affiliation>Department of Clinical and Social Dentistry, Federal University of Paraíba, João Pessoa, PB, Brazil.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Orthodontics and Pediatric Dentistry, Araraquara Dental School, State of São Paulo University, Araraquara, SP, Brazil.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimarães, Portugal.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, 4805-017, Portugal.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Babo</LastName>
<ForeName>Pedro S</ForeName>
<Initials>PS</Initials>
<AffiliationInfo><Affiliation>3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimarães, Portugal.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, 4805-017, Portugal.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Silva</LastName>
<ForeName>Cristiana R</ForeName>
<Initials>CR</Initials>
<AffiliationInfo><Affiliation>3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimarães, Portugal.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, 4805-017, Portugal.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Rodrigues</LastName>
<ForeName>Márcia T</ForeName>
<Initials>MT</Initials>
<AffiliationInfo><Affiliation>3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimarães, Portugal.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, 4805-017, Portugal.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hebling</LastName>
<ForeName>Josimeri</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Orthodontics and Pediatric Dentistry, Araraquara Dental School, State of São Paulo University, Araraquara, SP, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Reis</LastName>
<ForeName>Rui L</ForeName>
<Initials>RL</Initials>
<AffiliationInfo><Affiliation>3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimarães, Portugal.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, 4805-017, Portugal.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, 4805-017, Barco, Guimarães, Portugal.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gomes</LastName>
<ForeName>Manuela E</ForeName>
<Initials>ME</Initials>
<AffiliationInfo><Affiliation>3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017, Barco, Guimarães, Portugal. megomes@dep.uminho.pt.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, 4805-017, Portugal. megomes@dep.uminho.pt.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, 4805-017, Barco, Guimarães, Portugal. megomes@dep.uminho.pt.</Affiliation>
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<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>06</Month>
<Day>14</Day>
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Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation.

Hyaluronic acid hydrogels incorporating platelet lysate enhance human pulp cell proliferation and differentiation.

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